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HomeMy WebLinkAboutFebruary 03, 2014 AgendaAGENDA CITY OF DENTON CITY COUNCIL February 3, 2014 After determining that a quorum is present, the City Council of the City of Denton, Texas will convene in a Work Session on Monday, February 3, 2014 at 11:30 a.m. in the Council Work Session Room, 215 E. McKinney Street, Denton, Texas at which the following item will be considered: NOTE: A Work Session is used to explore matters of interest to one or more City Council Members or the City Manager for the purpose of giving staff direction into whether or not such matters should be placed on a future regular or special meeting of the Council for citizen input, City Council deliberation and formal City action. At a Work Session, the City Council generally receives informal and preliminary reports and information from City staff, officials, members of City committees, and the individual or organization proposing council action, if invited by City Council or City Manager to participate in the session. Participation by individuals and members of organizations invited to speak ceases when the Mayor announces the session is being closed to public input. Although Work Sessions are public meetings, and citizens have a legal right to attend, they are not public hearings, so citizens are not allowed to participate in the session unless invited to do so by the Mayor. Any citizen may supply to the City Council, prior to the beginning of the session, a written report regarding the citizen's opinion on the matter being explored. Should the Council direct the matter be placed on a regular meeting agenda, the staff will generally prepare a final report defining the proposed action, which will be made available to all citizens prior to the regular meeting at which citizen input is sought. The purpose of this procedure is to allow citizens attending the regular meeting the opportunity to hear the views of their fellow citizens without having to attend two meetings. 1. Receive a report, hold a discussion, and give staff direction concerning the 2013 mosquito season and upcoming 2014 season. NOTE: The City Council reserves the right to adjourn into a Closed Meeting on any item on its Open Meeting agenda consistent with Chapter 551 of the Texas Government Code, as amended, including without limitation, Sections 551.071 - 551.086 of the Texas Open Meetings Act. CERTIFICATE I certify that the above notice of meeting was posted on the bulletin board at the City Hall of the City of Denton, Texas, on the day of 2014 at o'clock (a.m.) (p.m.) CITY SECRETARY NOTE: THE CITY OF DENTON COUNCIL WORK SESSION ROOM IS ACCESSIBLE IN ACCORDANCE WITH THE AMERICANS WITH DISABILITIES ACT. THE CITY WILL PROVIDE SIGN LANGUAGE INTERPRETERS FOR THE HEARING IMPAIRED IF REQUESTED AT LEAST 48 HOURS IN ADVANCE OF THE SCHEDULED MEETING. PLEASE CALL THE CITY SECRETARY'S OFFICE AT 349 -8309 OR USE TELECOMMUNICATIONS DEVICES FOR THE DEAF (TDD) BY CALLING 1-800 - RELAY-TX SO THAT A SIGN LANGUAGE INTERPRETER CAN BE SCHEDULED THROUGH THE CITY SECRETARY'S OFFICE. AGENDA INFORMATION SHEET AGENDA DATE: Febwary 3, 2014 DEPARTMENT: Environmental Services and Sustainability ACM: Howard Martin, 349 -8232 14v— SUBJECT Receive a report, hold a discussion, and give staff direction concerning the 2013 mosquito season and upcoming 2014 season. BACKGROUND The City of Denton implemented the most extensive mosquito monitoring effort to date during the 2013 season. Monitoring began on April 14, 2013 and continued approximately weekly through November 19, 2013. Sixteen to twenty one traps were deployed each week, resulting in a total of 539 "routine monitoring" traps for the season. All mosquitoes collected during routine monitoring were shipped to the Texas Department of State Health Services (TDSHS) for species identification and West Nile Vines (WNV) screening. In addition to routine monitoring, the City of Denton and University of North Texas (UNT) researchers implemented a monitoring and testing program using the Rapid Analyte Measurement Platform (RAMP). The RAMP research was implemented as a pilot program to provide more rapid and flexible local West Nile Vines screening capabilities. As stated above, mosquitoes collected during routine monitoring events are shipped to the Texas Department of State Health Services (TDSHS) for species identification and WNV screening. The services provided by the TDSHS are extremely valuable to the overall monitoring program, but due to the State's workload can take a week or more before results are finalized. The RAMP approach allows local researchers to produce results within one day of collection. During the height of the mosquito season, the ability to quickly analyze mosquitoes for WNV can help facilitate much more rapid and flexible local responses. Monitoring for RAMP analyses began on May 7, 2013, and continued approximately weekly through November 5, 2013, resulting in a total of approximately 231 traps for the season. ,Surveillance and Response efforts Of the 770 total traps deployed, there were three samples collected that tested positive for WNV. The first positive samples were collected on October 1, 2013 at Robson Ranch, and represented one positive sample for routine monitoring and one for RAMP monitoring efforts. The third positive sample was collected at the Pecan Creek Water Reclamation facility on October 22, 2013. There were two human WNV cases reported in Denton during the 2013 season. The first case was reported on July 25t1' (onset date estimated as June 17t1') and the second case was reported on October 16th (onset date estimated as September 30th). RAMP monitoring was conducted in proximity to the human cases as soon as they were reported. The second human case in October resulted in the declaration of Risk Level 5 for the City and the subsequent decision to enact ground level spraying. The area surrounding the reported case was sprayed on th October 24 Page 2 of 5 During 2013, larviciding efforts within Denton were maintained at approximately the same level of effort used in the 2012 season. During 2013, a total of 7,677 BTI applications were made by Drainage, Parks, and Code enforcement personnel, and approximately 616 BTI briquettes were distributed to citizens. During 2012, a total of 7,386 BTI applications were made, and approximately 3,744 briquettes were provided to citizens. It is interesting to note that substantially fewer citizens obtained BTI from the City in 2013 when compared to 2012. Although somewhat speculative, this decrease is likely due to the much milder WNV season experience in 2013 and the resulting overall decrease in media coverage for the issue. During 2013, staff also implemented limited use of Natular larvicides when applications required a longer lasting alternative to BTL This larvicide appeared to work well for specialized applications. Following the 2012 season, Environmental Services staff worked with Technology Services to improve the mosquito trap and spraying maps available on the City of Denton web site. Maps used during the 2012 season were static images that did not allow users to effectively navigate within the map image, zoom into particular areas, or obtain information not originally included in the image. The previous maps also only indicated positive traps, and did not offer the capability to see all of the trapping locations assessed during the season. The revised mapping protocol used during 2013 now produces GIS based maps that offer navigation and zooming capabilities, and trap maps that show current trap results as well as all trap results (positive and negative) in selectable time sequences. The process by which addresses are extracted for automated phone notification during spray events has also been much improved through the use of the new system. ,Staff analyses of 2013 Mosquito Surveillance and Response Plan Following the 2012 season, staff worked extensively with the City Council Committee on the Environment and the City Council to revise the Mosquito Surveillance and Response Plan. Revisions to the plan were completed prior to the start of the 2013 season. Staff has reviewed the Plan following the 2013 season, and has made updates to statistics and some editorial corrections. Aside from these minor changes, staff recommends no additional changes to the plan. A copy of the 2014 Mosquito Surveillance and Response Plan is provided as Exhibit 2. Analysis of 2012 mosquito and human WNV data During the October 22, 2013 special called Council meeting to discuss Risk Level 5 and spraying, the City Council directed staff to analyze the 2012 WNV positive trap data and WNV positive human case data to determine if there were any relationships between trap results and human cases. These analyses can become quite complex, due in part to exposure assumptions, the time lag between human and mosquito results, and the need to establish some distance (proximity) between trap locations and positive human cases. For the issue of proximity, staff used a I mile distance surrounding the trap. The rationale for this approach is that the target mosquito (Culex quinquefasciatus) adults are known to be weak flyers and do not tend to move very far from the point of emergence. For the time lag issue, staff used a fairly simplistic approach by considering a WNV positive human case reported at any time during the season following a WNV positive trap as indicating a positive relationship. A similar approach was used for negative traps, which resulted in counts for all cases where a trap location was negative Page 3 of 5 all season and no human cases were reported within the distance criteria during the season. Although the total number of WNV positive human cases in Denton during 2012 was 36, it is important to note that not all positive human cases had a corresponding mosquito trap within one mile. Using this approach, the following contingency table was constricted Table 1. WNV Contingency Table There are a number of different statistical analyses that can be run with contingency tables in this form, most of which are some type of chi - square (or modified chi - square) analysis. Also, before discussing the results, it is important to point out that 2012 was the worst WNV season experienced in Denton, and the North Texas region was considered to be the epicenter of the 2012 WNV season for the entire United States. Even under these conditions, the incidence rate of Denton's population was still 0.03 %, or about 1 person out of 3,255 people. It is also important to point out that the trapping network was not set up with the intention of performing analyses like this, and the analyses have underlying statistical assumptions (such as randomness) that are questionable when applied to Denton's information. With this context in mind, the results can be summarized as follows. Using the information from Table 1 above, a person does have a significantly greater chance of contracting WNV if they live in proximity to mosquito trap that has tested positive for WNV when compared to someone who lives in proximity to a trap that has tested negative the entire season. The likelihood of someone contracting WNV is 14 to 73 percent higher, with an average of 59% higher, if they live in proximity to a positive trap when compared to someone living within proximity to a trap that was negative the entire season. In other words, assuming that in 2012 the incidence rate in the City of Denton for human WNV cases of 1 person in every 3,255 people being diagnosed with the disease represented the average incident rate in the City, then areas in proximity to a positive trap may have experienced an average incidence rate of 1 person in every 2,050 people. Current status of eyu1pment and inventory The City of Denton has two trailer mounted Ultra -low volume (ULV) sprayers for ground based spraying events. One sprayer is set up for continuous deployment, and one serves as a reserve in case of equipment failure. However, it is important to note that having two sprayers also provides the ability to conduct simultaneous spraying responses in different parts of the City. Although Denton has not had the need to conduct simultaneous events spraying to date, the capability exists to do so if needed. Because the 2013 season only had one spray event, the City of Denton currently has an inventory of Duet adulticide and associated cleaning solution that is sufficient for at least two large spray events. The current BTI inventory of 882 briquettes, however, will need to be replenished prior to the start of the 2014 season. Expenditures and bard et The amount spent on mosquito control activities during 2013 season was approximately $38,230. For comparison, 2012 total expenses were approximately $57,691. The majority of the difference between the two years was due to spraying activities, including the purchase of + Human case w/ in 1 mile No + human case w /in 1 mile Trap Positive 19 8 Trap Negative 1 8 There are a number of different statistical analyses that can be run with contingency tables in this form, most of which are some type of chi - square (or modified chi - square) analysis. Also, before discussing the results, it is important to point out that 2012 was the worst WNV season experienced in Denton, and the North Texas region was considered to be the epicenter of the 2012 WNV season for the entire United States. Even under these conditions, the incidence rate of Denton's population was still 0.03 %, or about 1 person out of 3,255 people. It is also important to point out that the trapping network was not set up with the intention of performing analyses like this, and the analyses have underlying statistical assumptions (such as randomness) that are questionable when applied to Denton's information. With this context in mind, the results can be summarized as follows. Using the information from Table 1 above, a person does have a significantly greater chance of contracting WNV if they live in proximity to mosquito trap that has tested positive for WNV when compared to someone who lives in proximity to a trap that has tested negative the entire season. The likelihood of someone contracting WNV is 14 to 73 percent higher, with an average of 59% higher, if they live in proximity to a positive trap when compared to someone living within proximity to a trap that was negative the entire season. In other words, assuming that in 2012 the incidence rate in the City of Denton for human WNV cases of 1 person in every 3,255 people being diagnosed with the disease represented the average incident rate in the City, then areas in proximity to a positive trap may have experienced an average incidence rate of 1 person in every 2,050 people. Current status of eyu1pment and inventory The City of Denton has two trailer mounted Ultra -low volume (ULV) sprayers for ground based spraying events. One sprayer is set up for continuous deployment, and one serves as a reserve in case of equipment failure. However, it is important to note that having two sprayers also provides the ability to conduct simultaneous spraying responses in different parts of the City. Although Denton has not had the need to conduct simultaneous events spraying to date, the capability exists to do so if needed. Because the 2013 season only had one spray event, the City of Denton currently has an inventory of Duet adulticide and associated cleaning solution that is sufficient for at least two large spray events. The current BTI inventory of 882 briquettes, however, will need to be replenished prior to the start of the 2014 season. Expenditures and bard et The amount spent on mosquito control activities during 2013 season was approximately $38,230. For comparison, 2012 total expenses were approximately $57,691. The majority of the difference between the two years was due to spraying activities, including the purchase of Page 4 of 5 sprayers, application costs, and chemicals. In addition, the RAMP pilot program was introduced in 2013, and required approximately $17,700 for the initial purchase of the RAMP instrumentation, associated equipment and reagents, and researcher funding. Staff recommends continuing the RAMP program in the upcoming 2014 season, and anticipates costs will be approximately $14,000. Estimating future costs for mosquito surveillance and response is difficult due to the uncertainties associated with spraying needs. Under the assumption of using the same level of surveillance, RAMP testing, and larviciding as in 2013, and adding 10 -12 large spray events, staff estimates that the following expenditures may be needed for the 2014 season. All expenses should be considered approximate. Table 2. 2014 Expenditures Estimates Description COST estimate UNT Monitoring Contract $16,125 UNT RAMP program $14,000 BTI purchase (6400 units) $5,000 Pesticide / Cleaning fluid (use chemicals on hand and plan for 10 new events) $3,000 Spraying contract ( approximately $300 -$500 per event, plan for 10 -12 events) use existing Sprayer re lacement fund (annual, based on replacement in 6 yrs = $16,600) $2,800 Sprayer and trailer license, calibration, maintenance, and fuel $1,000 TOTAL $41,925 Total fixed costs (UNT programs and BTI purchase) $35,125 Total variable / contingent costs (spraying 10 -12 large events 6,800 Staff suggests the following actions: • Maintain the agreement with UNT to conduct mosquito surveillance activities at the current level of effort (approximately $16,125). • Maintain Bti purchases and applications at current levels. • Continue the RAMP pilot program, including student support and reagent costs (approximately $14,000). • Maintain the Mosquito Surveillance and Response plan in its current form. • The current mosquito surveillance and response program is funded by Water and Wastewater budgets ($13,036 towards monitoring program). As outlined in Table 1, the total expenditure for the 2014 season under the assumption of 10 -13 spray events will require additional funding of approximately $28,889. If the spraying costs are not funded at the beginning of the season and are instead considered for funding on an "as needed" or emergency basis, the level of additional funding required will be approximately $22,000. • If funds are not fully expended for a given season, any remaining funds should be applied to the upcoming season (with the exception of the sprayer replacement funds, which should be allowed to accumulate until the estimated replacement cost is met). This approach will reduce the amount needed from contingency funds in a particular fiscal year if mosquito control activities were minimal in the previous season. Page 5 of 5 Staff recommends considering adding the "contingency funded" mosquito response activities outlined above as a formal supplemental budget item in the 2014 -2015 and subsequent budgets. OPTIONS 1. No further action 2. Direct staff to proceed with implementing suggestions as outlined above. 3. Table for future consideration RECOMMENDATION Staff recommends Option 2. PRIOR ACTION/REVIEW (Council, Boards, Commissions) None FISCAL INFORMATION The amount expended in the 2013 mosquito season was approximately $38,230. The estimated cost for the 2014 season, under an assumption of no spraying, is $35,125. The estimated cost for 2014 season is $41,925, under the assumption that 10 -12 large spray events will be needed. EXHIBIT 1. Presentation 2. Mosquito Surveillance and Response Plan, 2014 Final Draft Respectfully prepared and submitted by, Kenneth Banks. Director, Environmental Services and Utility Budgets EXHIBIT 1 City of Denton City Council February 3, 2014 2013 Season Snapshot • Routine monitoring — Started April 14, ended Nov. 19 — 16 -20 traps per week, 539 total for season • Rapid Analyte Measurement Program (RAMP) — Started May 7, ended Nov. 5 — 8 -10 traps per week, 231 total for season • Total of approximately 770 traps for the season • Three positive tests: Two at Robson Ranch Oct. 1, and one at Pecan Creek WRF Oct. 22 2013 Season Snapshot • Human cases: — Two reported, July 25 and October 16 — October 16 resulted in Risk Level 5 and special called Council session to consider spraying — Area around second case sprayed on October 24 • RAMP Pilot Program evaluation — Ability to analyze in 1 day (TDSHS 7+ days) — Allowed rapid monitoring around human cases — Provided additional monitoring and flexibility — Staff perspective: program successful 2013 Season Snapshot • Larviciding: — 7,677 BTI applications, some Natular — 616 briquettes provided to citizens — 2012 (comparison): 7,386 BTI, 3,744 to citizens • Public Information — Spray and Trap Maps in prior years were "static" — Maps improved for 2013, images are GIS, allow users to navigate, current and historical trap data available. Address and callout extraction is easier. — Public Communication provided excellent support. Mosquito Surveillance and Response Plan • Substantial changes discussed with Council following 2012 season • Changes implemented prior to 2013 season, and worked well • Staff has reviewed the plan and has updated statistics and made minor editorial changes • Staff recommends no changes for 2014 season Analysis of mosquito /human cases • During 10 -22 -13 Special Called meeting, council asked staff to analyze relationships between human cases and trap data for 2012 • Complications include exposure assumptions, spraying activity impacts, time lag, and trap distance. • Monitoring network was not designed for this kind of analysis, so possible violation of underlying assumptions of statistical methods (randomness, independence of variables, etc) Analysis of mosquito /human cases • Proximity issue — target mosquito is a weak flyer, used a distance of < 1 mile • Time lag — positive relationship if + result mosquito observed in a trap and human case was reported in proximity at any point during remainder of the season WNV Contingency table Trap positive 19 8 Trap negative 1 8 Results • In 2012, higher risk of contracting "V if one lived < 1 mile of a + trap, compared to living near a trap that was negative for all events • Likelihood was 14 — 73% higher, with an average of 59%. • Assuming 2012 average incident rate was 1 in 3,255, then 59% increase is 1 in 2,050 people • Caveat: this approach is "stretching" the statistical test assumptions of randomness and independence, and ignores spray effects Equipment and inventory status • 2 ULV sprayers — one in service, one backup. Units have been very reliable • Enough chemicals for 4 -5 spray events. • BTI inventory is low and will be replenished for next season Expenditures and Estimates 2012 $57,691 2013 $38,230 Description 2014 estimate UNT Monitoring Contract $16,125 UNT RAMP program $14,000 BTI purchase (6400 units) $5,000 Pesticide / Cleaning fluid (use chemicals on hand and plan for 10 new events) $3,000 Spraying contract ( approximately $300 -$500 per event, plan for 10 -12 events) use existing Sprayer replacement fund (replacement in 6 yrs = $16,600) $2,800 Sprayer and trailer license, calibration, maintenance, and fuel $1,000 TOTAL $41,925 Total fixed costs (UNT programs and BTI purchase) $35,125 Total variable / contingent costs (spraying 10 -12 large events) 6,800 Staff suggestions • Maintain UNT surveillance agreement • Continue BTI applications at current levels • Continue RAMP program • Maintain MSRP in current form • Secure additional funding needs through contingency, remaining funds used for subsequent years • Consider mosquito program for supplemental budget. Options 1. No further action 2. Direct staff to proceed with implementing suggestions 3. Table for future consideration • Staff recommended Option 2. Discussion EXHIBIT 2 Mosquito Surveillance and Response Plan City of Denton, Texas pupa female x v a, 'X a� eggs 2014 FINAL DRAFT prepared by Kenneth E. Banks, Ph.D. Department of Environmental Services and Sustainability City of Denton TABLE OF CONTENTS: 1.0 Introduction ........................................... ............................... 1 2.0 Objectives ............................................... ............................... 2 3.0 West Nile Vines Concerns ........................... ............................... 2 4.0 The Role of Residents .................................. ............................... 4 5.0 Denton's Plan for Mosquito Surveillance .......... ............................... 5 5.1 Adult mosquito collections .......... ............................... 6 6.0 Mosquito Control Strategies .............................. ............................... 6 6.1 Larviciding vs. adulticiding ........ ............................... 8 6.2 Rationale for different treatment methods ....................... 9 6.3 Integrated Pest Management ......... ............................... 9 7.0 Denton's Plan for Mosquito Control ................. ............................... 10 7.1 Adulticidmg .......................................... ............................... 10 7.1.1 Triggers for adulticiding ........... ............................... 10 7.1.2 When to use adulticides ............ ............................... 11 7.1.3 Where to use adulticides ........... ............................... 11 7.1.4 Human Population density considerations ................ 11 7.1.5 Mosquito population considerations ......................... 12 7.1.6 Local perspectives on spraying .. ............................... 12 7.1.7 Denton's plan for mosquito adulticiding operations . 12 7.2 Plan for Public Education Concerning Mosquitoes .............. 14 7.3 City of Denton Response Plan ............... ............................... 14 7.3.1 Risk Level 1 -Normal Response .............................. 15 73.2 Risk Level 2 - Enhanced Response ........................... 16 7.3.3 Risk Level 3 - Public Health Concern ....................... 17 73.4 Risk Level 4 - Public Health Warning ...................... 18 73.5 Risk Level 5 - Public Health Alert ......................... 19 References.................................................................. ............................... 21 1.0 INTRODUCTION Mosquitoes are insects that belong to the order Diptera, or Tree Flies. Female mosquitoes have modified mouthparts that form a long piercing - sucking proboscis, while male mosquitoes have mouthparts that are incapable of piercing skin. There are over 2,500 different species of mosquitoes that have been identified throughout the world, with approximately 150 species occurring in the United States. The Texas Department of Health estimates that there are approximately 82 -84 mosquito species in the State of Texas, although only about 12 of these mosquito species have been implicated in the transmission of serious diseases. Mosquitoes typically need still, stagnant water that is isolated from fish or other small predators to complete their metamorphosis from egg to adult. Larval habitats can range from marshes, freshwater wetlands, and tree holes to human -made strictures like catchments, drains, gutters, and discarded tires. Not all species feed on humans and other mammals, and many species feed mostly on birds, amphibians, or reptiles. Only a small percentage of the known mosquito species are considered to be diseases vectors. Although only a small percentage of mosquito species are capable of transmitting diseases, mosquitoes are still considered to be a very important vector for disease transmission. Within the United States, the occurrences of mosquito -borne illnesses have been relatively rare in recent years. However, epidemics of mosquito -borne diseases were once common in the United States. Outbreaks of Yellow Fever have been recorded as far north as Philadelphia during the Colonial Period, and Dengue fever was prevalent along the Gulf Coast until the mid- 1940s. Atone time, malaria was well established in the continental United States, especially in the south. Other mosquito - borne illnesses like LaCrosse, St. Louis, and Eastern Equine Encephalitis are still threats in certain areas of the country. Although many of these historical mosquito -borne diseases have been eliminated or at least controlled, the introduction and subsequent rapid spread of West Nile Vines within the United States is a topic of current concern. Mosquitoes may be controlled through a variety of different physical, chemical, and biological methods. Physical methods usually involve source reduction, which is simply the physical removal of mosquito breeding habitats. Biological measures mainly center on the use of bacteria that kill mosquito larvae or the use of natural mosquito predators. Chemical treatment typically involves the application of pesticides to attempt to control larva or adult mosquito populations. Mosquito control pesticides are applied by various means, depending on the type and size of the area being treated. No matter how pesticides are applied, however, pesticides have the potential to impact non - target species, including humans. Depending on the pesticides used, potential impacts may include acute or chronic toxicity, reproductive and / or developmental effects, and indirect effects through the food chain. The tests required for pesticides registration also may not address non - target impacts. Information regarding the effects of inert ingredients in pesticide formulations, food chain effects, multi- generational effects, and the interaction of specific pesticides with other chemicals in the environment may also be lacking. It is therefore important to realize that there are risks associated with the use of pesticides just as there are risks associated with the potential for human or animal infection by a mosquito -borne illness. The purpose of this response plan is to provide a systematic way to consider and balance the risks associated with mosquito control measures. 2.0 Objectives The City of Denton Mosquito Surveillance and Response Plan was developed to meet several objectives. Specifically, the Plan: • Provides guidelines and information on mosquito populations, prevalence of diseases, and control strategies; • Provides a systematic approach for utilizing mosquito sampling and human disease data to establish Risk Levels; • Establishes actions that will be undertaken for each Risk Level; • Provides municipal staff and elected officials with a decision - support system; • Outlines the roles and responsibilities of municipal staff and elected officials. 3.0 West Nile Virus (WNV) Concerns West Nile Vines was first recorded in North American during August 1999 shortly following the Center for Disease Control and Prevention (CDC) and the New York City Department of Health responses to an unusual outbreak of encephalitis in northern Queens, New York. The cause of the observed illnesses was determined to be West Nile virus. During 1999, approximately sixty people were diagnosed with West Nile virus and seven elderly residents died from the infection. During the summer of 2000, WNV activity was detected again in New York City and appeared to be spreading. Fourteen people in the City of New York were diagnosed with acute WNV infection, six were identified in New Jersey, and I was reported from Connecticut. Outside of New York, WNV was detected in 12 states and in the District of Columbia. During 2001, the virus appeared again in New York City, causing seven cases that required hospitalization. In 2001, a total of 66 human cases with nine fatalities were reported throughout the nation, mostly spread throughout the eastern United States. During 2002, there were 4,156 laboratory- positive human cases and 284 deaths. The number of cases increased dramatically in 2003 as the disease spread westward, with 9,862 cases and 264 deaths. After 2003, annual national case counts have ranged from a low of 712 during 2011 to a high of 5,674 in 2012. In 2012, the highest incident rates in the nation were observed in the North Texas region, including Denton County. The relatively rapid spread of West Nile virus and the increase in disease incidence indicates that WNV is permanently established in the United States. It is likely that the virus survives the winter either within birds that remain in the area or within mosquitoes that survive the winter. When spring returns, the virus recrudesces within the birds and is readily passed to early season mosquitoes. As mosquito populations increase, mosquitoes begin to feed more frequently on birds, causing an increasing number of birds 2 and mosquitoes that are infected. If environmental conditions are favorable for transmission, the virus will amplify to a theoretical point of spillover. At spillover, the virus can bridge out of the bird - mosquito cycle through mosquitoes that feed on birds, humans, and other animals. At the point of spillover, transmission to humans becomes more likely. Currently, mosquito control is the only practical method of protecting the human population from WNV inflection. There are no known specific treatments or cures for the disease caused by this virus, and vaccines are currently not available for public use. The virus also kills a variety of native and non - native birds, and may have impacts on other wildlife. Weather plays a major role in the prevalence of WNV, and the relatively warm winter and spring experienced in North Texas during 2011 to 2012 may have been a significant factor in the prevalence of the disease during the 2012 mosquito season. Understanding the types of mosquitoes in the area that transmit the disease is also a crucial consideration for directing control responses and public education activities. Since not all mosquitoes carry the virus, enacting mosquito control efforts that attempt to non - selectively reduce all mosquito populations will not necessarily reduce the risk of WNV. In fact, based on 10 years of mosquito monitoring and disease analyses in the City of Denton, there appears to be only one mosquito that serves as a vector for this disease. Approximately 99 percent of the WNV infected mosquitoes captured in Denton's monitoring program over the last 10 years have been Culex quinquefasciatus, commonly known as the southern house mosquito. The control efforts enacted by the City are therefore focused on this one particular type of mosquito. The past experience of numerous mosquito control districts suggests that a mosquito control program should be based on the principals of Integrated Pest Management (IPM). The principals of IPM are: • knowledge of mosquito biology and the epidemiology of the mosquito -borne diseases; • surveillance and monitoring efforts for the detection and status assessment of mosquito populations and / or mosquito -borne diseases; • a multifaceted prevention and control program comprised of a system of control tactics which are compatible with each other and which are proven effective; • continued program evaluations and updates to ensure that the best methods are being used to meet the prevention and control objectives of the program; and • continual education of the public to create awareness, understanding, and support. These general guidelines have been used to develop the threshold -level responses of this surveillance and response plan. 4.0 The Role of Residents Residents can play an important role in reducing the number of adult mosquitoes by eliminating standing water that may support the development of mosquito larva and pupa. For example, residents can properly dispose of discarded tires, cans, buckets, maintain pools correctly, unclog blocked gutters and drains, dump water from bird baths and pet dishes at least every 2 -3 days, ensure that air conditioning condensate is not pooling for several days, control irrigation so that standing water is not produced, irrigate so that runoff is not produced, and perform similar activities around homes and businesses. Water that cannot be eliminated should be treated with a biological mosquito larvicide such as Bacillus thuringiensis israelensis (Bti) in accordance with manufacturer's recommendations. Residents should be aware that Culex quinquefasciatus, the target mosquito for control, tends to prefer to lay its eggs in "artificial" sources of water such as those listed above, as opposed to laying eggs in wetland areas, streams, and ponds. Generally, this mosquito cannot fly long distances, so emerging adult mosquitoes often remain near the habitat they lived in as larva and pupa. Adults will rest during the daytime in vegetation, particularly in areas where humidity is high. Irrigated urban and suburban landscapes can offer excellent habitat, especially if there is standing water present. Underground storm drains that receive small amounts of runoff from irrigation are also good habitat for these mosquitoes. As a result, Culex quinquefasciatus tends to become more associated with urban and suburban areas during the summer months. Often these same areas will also have birth baths, bird feeders, trees, and other vegetation that serve to attract birds, especially during the drier times of the year when food and water become scarce. As a result, the opportunities for WNV disease amplification in urban and suburban areas can become more pronounced during summer months. The role of residents and business owners in controlling larval habitat is therefore a crucial component of reducing local WNV risk. 5.0 Mosquito Surveillance The risk of mosquito -borne diseases depends on the size of mosquito populations and the incidence rate of disease, as well as the likelihood of the mosquito population changing in response to climate factors. Although mosquito abundance can be estimated through collection of either immature or adult mosquitoes, adult mosquito abundance is a key factor contributing to the risk of virus transmission. For this reason, the City of Denton monitoring program primarily focuses on collecting adult mosquito populations for targeting control measures and gauging the potential for disease outbreak. The current mosquito surveillance program is a partnership between the City of Denton and the University of North Texas. This surveillance program collects adult mosquitoes through the use of both CDC light traps and gravid traps. Captured mosquitoes are sent to the Texas Department of State Health Services for testing. Each sample (sometime referred to as a "pool ") consists of female mosquitoes that are collected at a specific collection site, in one of the two types of trap. The information obtained from these surveillance 4 efforts is used to map mosquito populations, provide public information, and to determine the incidence of WNV or other arbovirus. The mosquito surveillance program allows analysts to map potential mosquito breeding grounds and areas of persistent disease. Using this information, more targeted efforts towards habitat disruption, source reduction, larviciding operations, and other control mechanisms are possible. An effective, broadly applied surveillance program can allow analysts to detect the presence of WNV of other mosquito -borne viruses during the amplification phase. If targeted mosquito controls are implemented at the amplification stage, the likelihood of bridging can be minimized, thus reducing the risk of human transmission. Depending on weather patterns and monitoring results, trapping can be expanded or contracted and the trapping season may be lengthened or shortened. If control measures are applied, trapping data may also be of some use for evaluating the effectiveness of control measures. The overall goal of the mosquito surveillance program is to use data on mosquito populations and mosquito virus infections rates to: • assess the threat of human disease; • determine the geographical areas of highest risk; • determine the need for intervention events, and the timing of these events; • identify larval habitats that are in need of targeted control; • monitor the effectiveness of control measures; and • develop a better understanding of transmission cycles and potential vector species. Adult surveillance methods should: Use both fixed and flexible trap locations if possible • Fixed positions allow for the development of a database so year to year comparisons are possible; • Flexible sites allow for responses to epidemiological and natural events. Use more than one trapping method (CDC light traps, gravid traps, etc...) o Testing data collected in the City of Denton's monitoring program suggests that if resource limitations require a choice between CDC light traps or gravid traps, gravid traps are preferred. These traps have been more effective in collecting Culex quinquefasciatus and more effective for establishing the prevalence of WNV. Account for different influencing factors, including: • Habitat size and diversity; • Resource availability; • Proximity to human population centers and / or recreational areas; and • Flight ranges of target mosquito vector(s). Advantages of using adult mosquito surveys include: • The ability to provide early evidence of viral activity in an area; • Helpful for determining if viral activity is local and / or restricted to a few areas; • Providing information on potential mosquito vector species • Providing estimates of vector species abundance and temporal prevalence; • Providing information on virus infection rates for different mosquito species; • Providing information on potential risks to humans and animals; • Providing baseline data that can be used to guide emergency controls. Disadvantages of using adult mosquito surveys include: • The amount of labor required, and associated expenses; • The need for substantial expertise to ensure proper collection and handling of mosquitoes; • The delays associated with receiving mosquito arbovirus screening results; • The fact that collectors may be at risk from mosquito bites (although using personal protection methods can minimize risks). 5.1 Adult mosquito collection The adult mosquito surveys implemented by the City of Denton in conjunction with the University of North Texas are designed to determine the relative abundance of various species present during the sampling period as well as the incidence of arthropod -borne virus /diseases within the captured specimens. Using this information, City of Denton personnel determines the need for various control measures, and can conduct more effective searches for larval breeding places, assess the extent of the problem, and potentially gauge the effectiveness of control measures. Reports concerning the incidence rates of arthropod -borne virus /diseases and the relative risks to citizens are also produced. Currently, collections are made using both CDC light traps and gravid traps deployed simultaneously. 6.0 Mosquito Control Strategies Human activities can greatly affect the ecology of mosquito populations. Large concentrations of people or animals, for example, can increase exposure rates and the probability of disease outbreak. The use of irrigation, development of drainage networks, elimination of mosquito predators, prevalence of improperly maintained birdbaths and other water holding containers can increase the numbers of certain types of mosquitoes. 6 Expanding international trade and travel has increased the chance of introducing new mosquito species into our areas, as has happened with the Asian tiger mosquito (Aedes albopictus). Mosquito control strategies have changed dramatically over the last few decades. Diesel oil, inorganic poisons, and source reduction using ditching operations were the basic tools of early mosquito control programs. Chlorinated hydrocarbons, organophosphates, pyrethroids, monomolecular oils, bacteria, and natural predators are more recent additions to mosquito control efforts. With the growth of ecological consciousness and environmental science, people began to realize the environmental damage that accompanied the use of broad - spectrum chemical control agents, particularly those that did not readily break down in nature. Concerns were also raised because many mosquito populations also appeared to develop resistance to the more commonly utilized chemical control agents. Over the past few years, major advances have been made in the areas of biological mosquito control. Biological control strategies may include using natural predators like Gambusia affinis (Mosquito fish), fungi, protozoans, round worms, flat worms, and bacterial agents such as Bacillus thuringiensis israelensis (Bti). Each biological control agent has certain benefits and restrictions. In order to use a biological control agent successfully, the applicator must have a basic knowledge of biology associated with the control agent. Some biological control mechanisms, for example, are limited by salinity, temperature, or organic pollution and some mosquito species are much more susceptible to specific types of biological control agents. All of these factors must be considered when choosing and applying biological control agents. The perfect pesticide is one that is easily applied, reasonably inexpensive, not toxic to non - target organisms, and that eliminates the pest quickly before it becomes a threat. Although no single pesticide can combine all of these factors, certain types of Bacillus bacteria have been developed into pesticides that are very close to the perfect pesticide model. Bacillus thuringiensis israelensis (Bti), for example, is a naturally occurring soil bacteria that produces a poison capable of killing mosquito larvae. Bti is considered ideal for mosquito management because of its specificity for mosquito larvae and because of the minimal to no toxicity to non - target organisms. These bacteria form asexual reproductive cells, called endospores, which enable them to survive in adverse conditions. The endospores of Bti also contain crystals of an insecticidal protein toxin called delta endotoxin. Once ingested by a mosquito, the alkaline conditions of the stomach dissolve the crystal and release the delta - endotoxin. The toxin has an affinity for the stomach wall lining causing the cells to first swell then rupture. When enough stomach cells burst, the mosquito larvae is unable to effectively digest food. Once affected, larvae stop eating and rapidly die. Currently, Bti is commercially available in powder, liquid, granular, capsule, and "briquette" formulations. Agencies applying pesticides directly to waters of the United States, or where deposition may enter waters of the United States, are required to obtain a National Pollutant Discharge Elimination System (NPDES) permit from the Texas Commission on 7 Environmental Quality (TCEQ). This permit is titled "General Permit to Authorize Point Source Discharge of Biological Pesticides and Chemical Pesticides That Leave a Residue in Water". Agencies must comply with all applicable provisions of this permit (see TCEQ General Permit TXG87000). 6.1 Larviciding vs. Adulticiding In the past, many mosquito control programs have relied heavily upon adult mosquito controls using chemical agents. In certain areas, routine mosquito spraying has been an integral component of control strategies. However, even near - continuous exposures to pesticides may not kill all mosquitoes. Those mosquitoes genetically able to resist higher pesticide concentrations may survive and pass on this resistance to future generations. Eventually, the pesticide becomes less effective as resistance increases in the mosquito populations. Resistance can be minimized through the "Management by Moderation" approach. Management by moderation is an attempt to prevent the onset of resistance by: • using doses that are no lower than the lowest recommended application rate to avoid genetic selection of resistance genes; • using less frequent applications; • using chemicals of short environmental persistence; • avoiding the use of slow release formulations; • avoiding using the same class of pesticides to control both adults and immature stages; • applying pesticides to only hot spots. Area -wide treatments should only be considered during imminent public health threats; • leaving certain generations, populations, or population segments untreated; and • establishing action thresholds that accentuate control mechanisms other than chemical control of adult mosquitoes. Although management by moderation is a viable means of minimizing resistance, there are other environmental and human health concerns associated with the application of pesticides for adult mosquito control. Routine mosquito spraying, for example, has the potential to expose the public to pesticides. Depending upon the pesticide utilized, effects on humans are possible and may be more likely for people who already suffer from asthma or other respiratory problems. If proper safety precautions are not followed, applicator personnel may also be in danger of overexposure. Managers must therefore decide whether mosquito populations and the related threat of disease transmission or the chemicals used to control mosquitoes represent the bigger threat to humans. All mosquitoes begin their lives in water. Prime breeding sites include discarded tires left outdoors, poorly maintained bird baths, clogged rain gutters, unused swimming pools and plastic wading pools, pet dishes, or any other container capable of holding water for more than a few days. Mosquito breeding can therefore be prevented by either eliminating the source of water (source reduction) or by killing larvae (larviciding). Larviciding programs use a combination of source reduction, biological, and possibly chemical measures to control mosquito larvae before they develop into biting adults. If properly implemented, this strategy can be the most effective, economical and safest method for mosquito control because mosquito larvae are minimized, thus reducing the need for adult mosquito control and subsequently reducing the impacts of control measures on non - target organisms. Larviciding programs also offer the opportunity to use biological controls, which minimizes the impacts of the control program on non - target organisms and lessens the risk of chemical exposures to the public. Using biological controls also minimizes the chance of pesticide resistance in the mosquito populations. Experience suggests that the most effective and economical way to reduce mosquito populations is by larval source reduction through a locally funded abatement program. The goal of this program should be to monitor mosquito populations and initiate controls before diseases are transmitted to humans or domestic animals (CDC, 2001). When larval control is not possible or more immediate control measures are needed, adult mosquito control may be required to diminish populations of infected mosquitoes and interrupt viral transmission. Adult mosquito control products may be applied using ground based equipment, fixed wing aircraft, or helicopters. In all cases, products should be applied in ultralow volume (ULV) formulations and doses, and should be comprised of pyrethroids. 6.2 Rationale for different treatment methods Chemical usage should not be viewed as a long -term control strategy, and should be only implemented when there are occasional episodes of heavy uncontrolled breeding concurrent with a high degree of public health risk. This strategy is most appropriate because prolonged use might lead to the development of resistance in mosquito populations, thereby limiting overall management options (Tabashnik, 1990). The most efficient and effective program is one in which mosquito larvae are prevented from becoming biting adults through the use of biological control agents. The bacterium Bti or the related bacterium Bacillus sphaericus (Bs.) are considered to be the among the most environmentally acceptable commercially available biological control agents because of their relative specificity for mosquitoes and negligible toxicity for vertebrates (Rishikesh et al., 1983). Larvivorous fish can also be a valuable component of an integrated control program, either alone or together with chemical control agents (Walton et al., 1990; Walton and Mulla, 1991; Reed et al., 1995). For these reasons, the City of Denton relies heavily on source reduction and larviciding for mosquito control. 9 6.3 Integrated Pest Management Integrated pest management dictates that control efforts should be dependent on threshold levels. This means simply that a certain defined risk needs to exist before particular control measures are recommended. Levels of risk are based on knowledge of mosquito biology, the epidemiology of the mosquito -borne diseases, and monitoring efforts for the status assessment of mosquitoes and / or mosquito -borne diseases. Risks levels are then used to design multi- tactic prevention and control program that are comprised of a system of tactics which are compatible with each other and which are proven for their effectiveness. Continual program evaluations and updates ensure that the best methods are being used to meet the prevention and control objectives of the program, and continued public education is used to create awareness, understanding, and support. Frequent mosquito population assessments allow analysts to map potential mosquito breeding grounds and determine overall disease transmission risks. Using this information, more targeted efforts towards habitat disruption, source reduction, larviciding operations, and other control mechanisms are possible. 7.0 Denton's Plan for Mosquito Control The primary objective of mosquito control is to decrease the risk of mosquito -borne human diseases. This objective should be accomplished by: • Stressing source reduction as a viable means of control, both by residents and on municipal properties, including enforcement actions for stagnant water located on private property; • Aggressively larviciding where such activities are feasible, practical, and likely to be effective. This includes providing limited supplies of larvicides to citizens for use on private property. • Promoting the use of personal mosquito protection measures, especially for the elderly and those individuals with compromised immune systems, through public education and outreach. • Providing public information so that citizens are informed about the current Risk Level, areas of the City where WNV has been located, current municipal control measures, and what can be done by the public to help reduce risks. • If warranted, implement adult mosquito control measures through targeted ULV pesticide applications (adulticiding). 7.1 Adulticiding Adulticiding should and will be considered a supplemental control measure. The decision to spray shall be based on the conditions listed (in no particular order) below. 7.1.1 Triggers for adulticides: Adulticiding shall be considered only when there is evidence of WNV activity at a level suggesting a high probability of human infection. In 10 general, finding an isolated WNV- positive mosquito pool does not by itself constitute evidence of an imminent threat to human health and does not warrant adulticiding. Physicians and laboratories are required by Title 25 of the Texas Administrative Code (Pt. 1, Ch. 97, Subch A, §973) to report cases of WNV infection or positive test results in humans to the State and County Health Departments. Positive human WNV cases are investigated by Health Department officials. During an investigation, the Health Department collects demographic and clinical information from the patient and attempts to determine date of onset, whether the infection was acquired locally or from a region outside of the patient's residence, and whether the infection may have been acquired by a non - mosquito route of transmission such as a blood transfusion or organ transplant. To help aid control measures, the Health Department provides some of this information to municipal officials within the municipality where the patient resides. Human case information is used by municipal officials as one of the trigger conditions for the risk levels outlined in this plan 7.1.2 When to use adulticides: The goal of spraying is to reduce the risk of human diseases by decreasing the number of target adult mosquitoes as much as possible. However, today's pesticides tend to only be effective when physically contacting the mosquito, which is most likely to happen when mosquitoes are actively flying. This typically will mean that spraying should be conducted between dusk and dawn. Since the target mosquito Culex quinquefasciatus, is thought to be most active soon after dusk, spraying should be timed to strike the best balance between impacting the target mosquito during its most active time and minimizing impacts on non - target organisms. It is also important to realize that weather conditions, including air temperature, have a large influence on the effectiveness of adulticides. Spray events should be cancelled and rescheduled if conditions are not conducive for effective spraying. 7.1.3 Where to use adulticides: The terrain of the proposed spraying area has a major impact on the pesticide effectiveness. If there is substantial vegetation, dense shrubbery, trees, or hedges, pesticide applications can be rendered ineffective. The density of houses and other physical obstructions can also influence the effectiveness of pesticides. Applications should be made so that the maximum penetration into these types of obstructions is achieved. In some cases, a mild breeze may be able to help distribute the pesticide. Application strategies should consider the meteorological conditions at the time of application, and use these conditions to help improve application effectiveness if possible. 7.1.4 Human population density considerations: The human population density in an area where there is evidence of intense epizootic activity should also be considered. If the area in question is rural and does not contain many people, the cost and potential risk associated with spraying may not justify its use. If the area in question is densely populated, adult mosquito control may be more justified as long as adequate precautionary measures are taken. 11 7.1.5 Mosquito population considerations: Information from mosquito surveillance can be helpful in determining when to conduct mosquito control and to assess the effectiveness of control measures. It is also important to know the numbers and species of the vector populations in specific localities. The best way to obtain this information is through mosquito trapping efforts. The City of Denton formed a partnership with the University of North Texas to provide the specially trained staff needed for systematic mosquito trapping efforts. While trapping efforts are crucial for assessing mosquito populations and the prevalence of disease, it is important to realize that surveillance specimens requires some processing time after collection. In the time between the collection date and the date of test results, circumstances may have occurred which would alter a decision to spray. For example, weather conditions may have adversely affected mosquito populations, local mosquito habitats may have been altered, or larviciding efforts may have reduced the number of newly emerged adults. All of these occurrences may result in a reduced need to spray, and should be taken into consideration during control efforts. Surveillance information should also be evaluated through time to determine the progress of diseases and the relative risk of disease transmission. Surveillance information may also be of some use in gauging the effectiveness of control measures, although establishing control effectiveness is difficult using only surveillance information. In all cases, the available surveillance information should be evaluated as a part of the decision making process for enacting adulticidal activities. 7.1.6 Local perspectives on spraying: Different communities have different perspectives on the benefits and risks associated with adulticiding activities. While these perspectives are valid and should be considered, individuals are likely to have strong opinions on either side of the issue. The City of Denton has incorporated a rationale framework within the mosquito control program that is designed to be protective of both human health and the environment. The decision to spray, however, is a complex issue that will likely be faced without complete information. Thus, there will be citizens that do not believe that the City of Denton has done a good job with regards to reducing public health and environmental risks, regardless of the decision. The mosquito control program should therefore remain flexible and should attempt to address citizen complaints through public education and dialogue. 7.1.7 Denton's plan for mosquito adulticiding operations Once arbovirus activity is detected and the decision is made to implement mosquito control using adulticides, the size of the area to treat must be determined. Unfortunately, there is no simple formula for determining how large of an area to treat, nor is there adequate information to guide decisions about the degree of vector population suppression that must be attained, or for how long this suppression must be maintained to reduce the risk of disease. The CDC (2001) suggests considering the following factors where deciding the scope of the adulticiding effort: 12 • the general ecology of the area; • the flight range of vectors that are known or are believed to be of importance in the area; • the population density of the vectors; • the length of time since virus- positive mosquito pools were collected; • the potential risk to the human population (including the age demographics of the area) as well as the community perception of the relative risk of pesticides versus the risk of arbovirus infection; and • the season of the year - how much time the transmission risk can be expected to persist until the vectors enter diapause; It is very likely that some of these factors will be unknown or poorly known, and practical experience in conducting a mosquito control program is needed to refine control recommendations. If adulticiding operations are conducted, the following parameters should be monitored: • Weather conditions during the application (temperature, wind speed, wind direction, etc...); • Flow rate of Ultra -Low Volume applications; and • Overall amount of pesticide applied. If the application of adulticides is deemed necessary, the public must be informed. The following actions / activities will take place prior to adulticide applications: • Information will be released 24 hours in advance through the media and through the city of Denton web site. Under certain conditions in Public Park areas, applications may take place in less than 24 hours notice. For these cases, the facility will be closed to the public during and a few hours after application. • Press releases should be prepared and shared with the appropriate media • CodeRed, the Customer Service automated calling systems, and available forms of social media should be used to inform citizens of spray events. CodeRed will make an automated phone call with a message to all individuals that have signed up for the service. The Customer Service automated calling system will make an automated phone call with a message to all individuals within an area designed by the spray map. For both systems, it is possible that some residents outside of the spray area will receive one of these calls. However, these systems are the most rapid and accurate means of notifying the public of upcoming spray events. • If practical, street signs indicating that spraying will occur in the neighborhood should be placed at major roadways entering and exiting the targeted area. 13 7.2 Plan for Public Education Concerning Mosquitoes Public education is a key component of a successful mosquito control program. Since the appearance of the West Nile Vines in the United States, the City of Denton has produced and aired many public service announcements, given numerous presentations, and provides information on the City web page concerning this disease. Depending somewhat on Risk level, the following key information will be conveyed to the public: • The location of WNV positive mosquito trap locations and current risk level; • Comprehensive prevention strategies and activities used by the City of Denton to address the threat of West Nile Vines; • The public will be advised to eliminate standing water sites by removing all materials that can hold water for longer than 2 -3 days; and • The public will be informed about the symptoms of West Nile Vines (headache, high fever, muscle pain, weakness, and disorientation). Techniques used to disseminate information may include any or all of the following: • Televised public service announcements using Denton's local cable channel; • Radio announcements; • Brochures for public use placed in municipal buildings; • Postings describing the current risk level placed in municipal buildings; • Brochures and / or fact sheets to be distributed to community -based organizations, community boards, elected officials, schools, nursing homes, libraries, outdoor activity sites, etc.; • Presentations to elected officials and / or community groups concerning the current risk level, mosquito population and disease status, and mosquito control activities; and • Press releases describing West Nile virus response activities. 7.3 City of Denton Response Plan The purpose of this response plan is to: I. Minimize human illness through public education and targeted vector control. 2. Provide a semi- quantitative measure of virus transmission risk to humans that can be used to plan and adjust control activities. 3. Map the density of mosquitoes and the incidence of the virus within the City of Denton. 4. Identify areas where the incidence of disease is high and provide appropriate warnings to the citizens of Denton. 5. Identify the key vector species that carry diseases within Denton. 14 The prevention of West Nile virus, as with many diseases, is most efficiently accomplished by ensuring that prompt, accurate information reaches the public. If the appropriate information reaches the public in a timely manner, personal protective measures may be implemented, and control strategies can be enacted efficiently. The City of Denton will provide continuous information on the city web page concerning West Nile virus, including a frequently asked questions (FAQs) section, disease symptoms, personal preventative measures, and sources of additional information. If a sampled mosquito pool tests positive for arthropod -borne virus /diseases, information describing the location of the sampling event, the date, and other pertinent information will be provided. This response plan is partially based on Texas Department of Health recommendations. The plan is divided into 5 levels based on the risk of human disease. Each risk level is described below, along with specific recommended responses. In accordance with the principals of Integrated Pest Management Control measures are recommended for each level. Generally, there are 4 surveillance / observational factors that are evaluated to determine if the trigger condition has been met for a particular risk level. These factors are: I. Environmental / climatic conditions (mainly temperature, rainfall, and season) 2. Adult Odex quinquefasciatus abundance 3. Virus infection rates in Culex quinquefasciatus and other mosquito vectors 4 Confirmed infections in humans, including epidemiological information. 7.3.1 Risk level 1 - Normal Response Condition: Probability of human outbreak is none or remote Trigger: Normal mosquito activity with no evidence of arthropod -borne virus /disease detected during the past 3 years in vectors, humans, or other hosts. Recommended Response: ,Surveillance: Surveillance is optional. Under the normal response, mosquitoes are considered to be only a nuisance without significant influences on public health. Complaint calls and informal surveys of larva and adults will be used to direct mosquito control efforts. Public Iuf )rmatiou Education: Publicize methods for mosquito reduction and personal protection prior to the main season for mosquito activity and outdoor human activities. 15 Control Measures Use public information to promote source reduction and personal protection. Conduct standard larviciding approaches using Bacillus thuringiensis israelensis (Bti) . Rationale: Larviciding and source reduction / elimination are considered to be the most effective long -term solutions for mosquito control. The control measures are designed to accomplish mosquito control by preventing larval mosquitoes from becoming biting adults. 7.3.2 Risk Level 2 - Enhanced Response Condition: Probability of human outbreak is low Trigger: Normal mosquito activity with little or no evidence of arthropod -borne virus /disease. Enhanced response level is due to recent historical presence of arthropod -borne virus /disease in vectors, humans, or other hosts within the vicinity of Denton (approximately 100 miles). Recommended Response: ,Surveillance: Surveillance is optional, but strongly encouraged. If possible, conduct routine surveillance of adult mosquito populations using light traps baited with CO2 and gravid traps (at minimum). Collected mosquito species should be identified to species level and mosquito pools should be screened for the presence of arboviruses. If positive tests are obtained during arthropod -borne virus /disease screening, additional tests should be performed to determine what type of arbovirus is present. Public Iuf )rmatiou Education: Implement all steps for public information established by the Public Communication Office for this Risk Level. Generally, this will reflect routine public information concerning methods for mosquito reduction and the importance of personal protection measures. This information should be disseminated at the beginning of the mosquito season. Control Measures Use public information to promote source reduction and personal protection. Conduct standard larviciding approaches using Bacillus thuringiensis israelensis (Bti). Rationale: Larviciding and source reduction / elimination are considered to be the most effective long -term solutions for mosquito control. The control measures are designed to accomplish mosquito control by preventing larval mosquitoes from becoming biting adults. 16 7.3.3 Risk Level 3 - Public Health Concern Condition: Probability of human outbreak is low to moderate Trigger: Arthropod -borne virus /disease isolated from mosquitoes collected during trapping activities at a single monitoring site. Recommended Response: ,Surveillance: Continue to conduct routine surveillance of mosquito populations. If resources allow, increase surveillance in the area where the positive sample was collected. Use geographic information systems to plot the location of the positive sample and provide this information to the public via the City of Denton web page. Public Iuf )rmatiou Education: Implement all steps for public information established by the Public Communication Office for this Risk Level, which includes public notification, press releases, and City of Denton web page updates with a map of the positive trap location. Notify medical professionals, veterinarians, and all those registered with the City of Denton's various public notification options of the presence of the disease. Control Measures Use public information to promote source reduction and personal protection. Conduct enhanced larviciding using Bacillus thuringiensis israelensis (Bti). Increase efforts in areas where positive mosquito pools were detected. Control measures will be implemented based on the following IPM criteria: time of year, the extent of previous mosquito control activities, the current level of mosquito activity, weather conditions, and surveillance results. Prepare for ground level spraying activities using ULV applications of pyrethriods. Ensure adequate funding and personnel are in place for conducting ULV applications. Rationale: Larviciding and source reduction / elimination are considered to be the most effective long -term solution for mosquito control. Increasing these measures in areas where positive mosquito pools are detected offers an additional degree of risk reduction. 7.3.4 Risk Level 4 - Public Health Warning Condition: Probability of human outbreak is moderate to high Trigger: Multiple mosquito pools collected at different times and locations test positive for arthropod -borne virus /diseases. Single human case confirmed with laboratory testing 17 Recommended Response: ,Surveillance: Continue to conduct routine surveillance of mosquito populations. Increase surveillance in areas where WNV positive samples have been collected if resources are available. Use geographic information systems to plot the location of positive mosquito samples and provide this information to the public via the City of Denton web page. Public lqf )rmation Education: • Implement all steps for public information established by the Public Communication Office for this Risk Level, which includes public notification, press releases, and City of Denton web page updates with a map of the WNV positive trap location(s). • Update web page map for all subsequent WNV positive trap locations • Notify medical professionals, veterinarians, public health officials, and all those registered with the City of Denton's various public notification options of the presence of the disease. Control Measures Use public information to promote source reduction and personal protection. Intensify enhanced larviciding efforts using Bti in targeted areas. Consider using Spinosad products in areas where positive mosquito pools were detected, if applicable. Control measures will be implemented based on the following IPM criteria: time of year, the extent of previous mosquito control activities, the current level of mosquito activity, weather conditions, and surveillance results. Finalize all actions needed for implementing ground level spraying activities using ULV applications of pyrethriods Rationale: Larviciding and source reduction / elimination are considered to be the most effective long -term solutions for mosquito control. Increasing these measures in areas where positive mosquito pools are detected offers an additional degree of risk reduction. 7.3.5 Risk Level 5 - Public Health Alert Condition: Human outbreak is confirmed Trigger: Multiple human cases. Cases must be confirmed by laboratory testing. IN Recommended Response: ,Surveillance: Continue to conduct routine surveillance of mosquito populations. If resources allow, increase surveillance in areas were arthropod -borne virus /disease positive samples were collected. Use geographic information systems to plot the location of WNV positive mosquito samples, and provide this information to the public. Public lqf )rmation Education: • Implement all steps for public information established by the Public Communication Office for this Risk Level, which includes public notification, press releases, and City of Denton web page updates with a map of the WNV positive trap location(s). • Update web page map for all subsequent WNV positive trap locations • Notify medical professionals, veterinarians, public health officials, and all those registered with the City of Denton's various public notification options of the presence of the disease. • Increase public education, emphasizing source reduction, personal protection, and disease symptoms. Control Measures: Use public information to promote source reduction and personal protection. Intensify larviciding efforts using Bti in targeted areas. Additional larvicides containing IGRs or pesticides such as Spinosad may be applied at this time. Intensely treat standing water in City Parks. If the threat to human health is considered imminent, consider targeted adulticide applications using ground -based Ultra -Low Volume (ULV) equipment. The decision to spray will be made by a Resolution of the City Council of Denton prior to the first application, for each mosquito season. Based on information available at the time of the Council decision, the Resolution may include specific requirements in addition those outlined in this plan. ULV applications should be implemented in the vicinity (approximately 1 mile) of areas where positive human cases were detected. When ULV applications are initiated, notification will be provided to the public as outlined in Section 7.1. During 2012, the City of Denton utilized Duet, a synthetic pyrethroid product with an additional chemical designed to induce "benign agitation" of mosquitoes. The purpose of benign agitation is to increase the likelihood of mosquitoes flying during the application, which increases application effectiveness. The Duet product is also mineral oil based, versus water based, which likely increases the compound's effectiveness in the higher air temperatures typical during summer applications. Mineral oil based compounds may also be less likely to affect non - target organisms in aquatic environments because of the lack of solubility of the oil based compound in water. Control measures will be implemented based on the following IPM criteria: time of year, the extent of previous mosquito control activities, the current level of mosquito activity, 19 weather conditions, the species of mosquitoes that test positive for disease, the number of local mosquito pools which test positively for disease, the likely time until a killing frost, the density of roads or other access to mosquito breeding grounds, and the density of human populations. Epidemiological data associated with reported human cases of WNV will also be considered. In general, if an area has been sprayed, that area will not be sprayed again in response to an additional human case unless the date of onset of the new case indicates exposure occurred after the previous spraying event. Areas will also not be sprayed in response to a reported human case if epidemiological information clearly indicates that exposure did not occur in the City of Denton. If public health emergencies are declared at the county or state level, the recommended responses associated with the declaration will take precedence over the control plan of the City of Denton. Rationale: Larviciding and source reduction /elimination are considered to be the most effective long -term solutions for mosquito control. Increasing these measures in areas where positive mosquito pools are detected offers an additional degree of risk reduction. However, at this Risk Level, applications of adulticides may be needed to rapidly reduce mosquito populations and halt disease transmission. The objective is to kill a high enough proportion of older adult mosquitoes to break the disease transmission cycle. If adult mosquito controls are implemented, the approaches for adulticiding outlined in this plan are effective and are designed to minimize adverse effects to the environment and non - target organisms. 20 References: Center for disease control (CDC). 2001. Epidemic/ Epizootic West Nile Vines in the United States: Revised Guidelines for Surveillance, Prevention, and Control. Proceedings from a Workshop Held in Charlotte, North Carolina. January 31- February 4, 2001. Reed, S.C., Cites, R.W. and E.J. Middlebrooks. 1995. Natural systems for waste management and treatment. McGraw -Hill, New York, USA. Rishikesh, N., Burgess, H.D., and Valdekar, M. 1983. Operational use of Bacillus thuringiensis serotype H -14 and environmental safety. WHO /VBC/83.871, World Health Organization, Geneva. Tabashnik, B.E. 1990. Modeling and evaluation of resistance management tactics. In Roush, R.T., Tabashnik, B.E. (Eds.), Pesticide Resistance in Arthropods. Chapman and Hall, New York, USA. Walton, W.E., Mulla, M.S., Wargo, M.J., and S.L. Durso. 1990. Efficacy of a microbial insecticide and larvivorous fish against Culex tarsalis in duck club ponds in southern California. Proceedings of the California Mosquito T'ector Control Association 58:148 -156. Walton, W.E. and M.S. Mulla, 1991. Integrated control of Culex tarsalis larvae using Bacillus sphaericus and Gambusia affinis: effects on mosquitoes and non - target organisms in field mesocosms. Bulletin of the ,Society for T'ector Ecology 16:203- 221. 21